The kinetics of salicylate hydroxylase reaction
نویسندگان
چکیده
منابع مشابه
Mechanism of the salicylate hydroxylase reaction.
1. A stable ternary complex of salicylate hydroxylase apoenzyme, flavin adenine dinucleotide, and salicylate was detected by a change of the absorption spectrum and by titration of the holoenzyme with salicylate. 2. The complex was enzymatically active, since the stoichiometric formation of the product, catechol, was observed upon introduction of air into a solution of reduced form of the compl...
متن کاملThe kinetic mechanism of salicylate hydroxylase as studied by initial rate measurement, rapid reaction kinetics, and isotope effects.
The kinetic mechanism of Pseudomonas cepacia salicylate hydroxylase has been examined by steady state initial rate measurements, and stopped flow and equilibrium studies. Results indicate that salicylate and NADH bind to the hydroxylase randomly. The enzyme is reduced and NAD+ is released. Oxygen subsequently binds to the reduced enzyme . substrate complex, leading to the production of hydroxyl...
متن کاملQuantification of salicylate in serum by use of salicylate hydroxylase.
In this enzymatic method for salicylate in serum, Pseudomonas salicylate hydroxylase (EC 1.14.13.1) is used. This stable enzyme catalyzes the stoichiometric, unidirectional conversion of salicylate and NAD(P)H to catechol and NAD(P)+ in the presence of molecular oxygen. The concentration of salicylate in a clinical sample is determined by measuring the delta A at 340 nm as compared with a stand...
متن کاملStudies of a Flavoprotein, Salicylate Hydroxylase
A flavoprotein, salicylate hydroxylase (salicylate, DPNH : oxygen oxidoreductase (I -hydroxylation, l-decarboxylating)), has been induced and isolated from a soil bacterium grown on salicylate as sole carbon source. In contrast to a similar enzyme studied by YAMAMOTO, S., KATAGIRI, M., MAENO, H., AND HAYAISHI, 0. ((1965) J. Bid, Chem. 240, 3408) this enzyme is dimeric with two subunits and two ...
متن کاملThe Gas Phase Oxidation of Acetaldehyde Reaction Mechanism and Kinetics
The mechanism of the low temperature oxidation of gaseous acetaldehyde was investigated in the temperature range of 1 50-400?°C. The minor, intermediate and major products were identified and measured quantitatively by sampling directly into the ionization chamber of an MS10-C2 mass spectrometer from the reactor. The formation of H2O, CO, CO2, HCOOH, H2, HCHO, CH3COOH and CH3OH as the major pro...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1970
ISSN: 0014-5793
DOI: 10.1016/0014-5793(70)80084-9